1. To determine whether the constructed plasmid
works well, we first transfected the plasmid with liposomes into cultured SH-SY5Y cells for 24 h and used western blot analysis with antibody against EGFP to check the expression of corresponding proteins.
We screened a pool of Arabidospis lines transformed with the activation vector for short hypocotyl mutants under dim far-red light . Consequently we isolated three mutant lines designated chibi1-3 (chi) .
3. Rats were
killed at different time point of reperfusion. Frontal and parietal lobe cortex o n the left hemisphere (6 to 11 mm to the tip of olfactory bulb) were separated and saved in li guid nitrogen.